The women were heading eastbound down the steep descent toward Kathu when their motorbike struck the front of a Toyota pickup truck heading up the hill at about 6:30am, reported Kusoldharm rescue workers.
The accident occurred in front of the Patong City sign popular with tourists for taking souvenir photos.
Both of the women remained conscious as rescue workers sped them to hospital.
One of the women suffered a broken leg, rescue workers reported.
Police have yet to report any findings in their initial investigation.