Here is a look at the musical highlights of the night:
Pop provocateur Janelle Monae and queen of sass Cardi B stunned with show-stopping numbers celebrating female sexuality.
Monae, whose futurist blend of pop and psychedelic funk scored her a nomination for Album of the Year, brought a girl gang to the stage to perform the Prince-influenced “Make Me Feel”.
Some of her dancers sported the iconic pink vagina pants from her video "Pynk", which nabbed a nomination for Best Music Video.
“Let the vagina have a monologue,” the genre-bending performer said – a lyric from her song “Django Jane”.
But it could have been the motto of the night.
Queen Cardi delivered a sexually charged rendition of her hit “Money”, sporting leopard thigh-highs, peacock-style feathers and a flapper-esque wig as she gyrated for the crowd on a rhinestone-encrusted piano.
Among those wowed by the performance from the 26-year-old rapper – who made history with her win for Best Rap Album – was rapper Offset, with whom Cardi shares a child.
The pair – who had recently separated – appeared to be going strong on the red carpet, where they lovingly touched tongues for the paparazzi.
Lady Gaga won three awards on the night, two of them for “Shallow”, the signature song from her blockbuster film “A Star Is Born”. When the moment came for her to belt out the song, she did not disappoint.
Gaga – sporting the glittering cat suit of a pop diva, not the mousy brown hair of her ingenue character in the movie – was without her duet partner, Bradley Cooper, who was in London attending Britain’s BAFTA film awards.
So she teamed up with her fellow songwriters including Mark Ronson to deliver the tune – high on dramatics, high on energy. When she belted out a guttural wail mid-song, the audience cheered.
Rising Cuban-American star Camila Cabello kicked off the glamour-dripping night with a colorful performance of her sultry hit “Havana”.
The Cuban-American raised in Miami was joined by rapper Young Thug, Colombian Latin trapper J Balvin and iconic trumpeter Arturo Sandoval for the infectious performance, which offered a bilingual portrait of diversity reflecting the massive influence of Latin music on the US charts.
And Jennifer Lopez wowed the audience with her exuberant ode to Motown that included inflections of salsa.
Parton, who was honored at a pre-gala event as the MusiCares Person of the Year for her philanthropic contributions, joined forces with a constellation of singers including her goddaughter Miley Cyrus and pop star Katy Perry.
The most memorable part of the medley came when Parton and Cyrus belted out “Jolene”, before Parton and others joined forces on her mega-hit “9 to 5”.
A powerful tribute to the late “Queen of Soul” Aretha Franklin saw Yolanda Adams, Fantasia Barrino and Andra Day – who donned garters and a feathered mini skirt – slay the late Queen of Soul’s classic hit “(You Make Me Feel Like) A Natural Woman”, moving the crowd to its feet.