The latest fatality was a navy squadron leader, reports the Bangkok Post.
Cdr Phalitdet Soomngern, 40, was pronounced dead at Siriraj Hospital, media reported. He was the leader of Fleet 201 at Wing 2 of the Naval Aviation Division in Rayong province.
His family had earlier sought blood donations to support him during surgery at the hospital.
Mountain B pub in Sattahip district, Chon Buri, caught fire about 1am last Friday.
Sixteen other injured people remain in hospitals. Many have extensive burns and are dependent on ventilators.
The death toll had increased to 16 on Wednesday.